Journal: iScience

Article Title: The role of epithelial progesterone receptor isoforms in embryo implantation

doi: 10.1016/j.isci.2021.103487

Figure Lengend Snippet: Key resources table

Article Snippet: In vitro HEC1A cell culture The human endometrial cancer cell line HEC1A was purchased from the ATCC (HTB-112, ATCC).

Techniques: Immunohistochemistry-IF, Western Blot, Plasmid Preparation, Recombinant, Modification, Electrophoresis, Multiplex Assay, Bicinchoninic Acid Protein Assay, Staining, SYBR Green Assay, Amplification, Purification, Generated, Software, Magnetic Cell Separation

Journal: iScience

Article Title: The role of epithelial progesterone receptor isoforms in embryo implantation

doi: 10.1016/j.isci.2021.103487

Figure Lengend Snippet: Suppressed LIF transcription by blocking ESR1 binding at Lif promoter (A–E) The 3D representative images of uterine glands in the adult Wnt7acre, ePRAoe, and ePRBoe mice at diestrus stage. Blue is non-cystic gland, and red is cystic gland (A). Real-time PCR showed mRNA levels of Lif and its receptors Lifr and IL6st in the whole uterus of the Wnt7acre, ePRAoe, and ePRBoe mice at D3.5 (B). Immunohistochemistry pictures of STAT3, pSTAT3 (Tyr705), ESR1, and PGR in Wnt7acre, ePRAoe, and ePRBoe mouse uterus at D3.5 (C). UCSC Genome Browser display of signals of ESR1 ChIP-seq of wild-type adult mouse uterus after 1-h E2 or vehicle treatment; PGR ChIP-seq of Pgrcre/+, Pgrcre/+PgrALsL/+, and Pgrcre/+PgrBLsL/+ uterus after 1-h P4 treatment; and H3K27AC ChIP-seq of D3.5 wild-type mouse uterus at Lif promoter (D). ChIP-qPCR indicates enrichment fold of ESR1 binding at the LIF promoter in the ePRAoe and ePRBoe mice at D3.5 (E). Scale bars: 100 μm in (A) and 50 μm in (C). Each dot indicates one mouse; the red line is median level. Student's t test. ∗p < 0.05. N = 6 mice for real-time PCR. N = 3 mice for ChIP-qPCR. See also Figure S6.

Article Snippet: Rabbit monoclonal pSTAT3 (Tyr705, IHC 1:400) , Cell Signaling , Cat# 9145; RRID: AB_2491009.

Techniques: Blocking Assay, Binding Assay, Real-time Polymerase Chain Reaction, Immunohistochemistry, ChIP-sequencing, ChIP-qPCR

Journal: iScience

Article Title: The role of epithelial progesterone receptor isoforms in embryo implantation

doi: 10.1016/j.isci.2021.103487

Figure Lengend Snippet: Key resources table

Article Snippet: Rabbit monoclonal pSTAT3 (Tyr705, IHC 1:400) , Cell Signaling , Cat# 9145; RRID: AB_2491009.

Techniques: Immunohistochemistry-IF, Western Blot, RNAscope, Plasmid Preparation, Recombinant, Modification, Electrophoresis, Multiplex Assay, Bicinchoninic Acid Protein Assay, Staining, Reverse Transcription, SYBR Green Assay, RNA Amplification, Purification, Generated, Software, Magnetic Cell Separation

Journal: iScience

Article Title: Methods to separate nuclear soluble fractions reflecting localizations in living cells

doi: 10.1016/j.isci.2021.103503

Figure Lengend Snippet: Key resources table

Article Snippet: mouse anti-BLVRB , Santa Cruz Biotechnology , Cat# sc-373692; RRID: AB_10918279.

Techniques: Recombinant, Protease Inhibitor, Plasmid Preparation, Magnetic Beads, Bicinchoninic Acid Protein Assay, Multiplex sample analysis, Multiplex Assay, Extraction, Software, Microscopy